Preserving Airway Smooth Muscle Contraction in Precision-Cut Lung Slices
نویسندگان
چکیده
منابع مشابه
Airway Smooth Muscle Sensitivity to Methacholine in Precision-Cut Lung Slices (PCLS) from Ovalbumin-induced Asthmatic Mice
Asthma is a chronic inflammatory disease characterized by airway hyperresponsiveness (AHR) and reversible airway obstruction. Methacholine (MCh) is widely used in broncho-provocation test to evaluate airway resistance. For experimental investigation, ovalbumin-induced sensitization is frequently used in rodents (Ova-asthma). However, albeit the inflammatory histology and AHR in vivo, it remains...
متن کاملATP stimulates Ca oscillations and contraction in airway smooth muscle cells of mouse lung slices
Bergner, Albrecht, and Michael J. Sanderson. ATP stimulates Ca2 oscillations and contraction in airway smooth muscle cells of mouse lung slices. Am J Physiol Lung Cell Mol Physiol 283: L1271–L1279, 2002. First published August 2, 2002; 10.1152/ajplung.00139.2002.—In airway smooth muscle cells (SMCs) from mouse lung slices, 10 M ATP induced Ca2 oscillations that were accompanied by airway contra...
متن کاملATP stimulates Ca2+ oscillations and contraction in airway smooth muscle cells of mouse lung slices.
In airway smooth muscle cells (SMCs) from mouse lung slices, > or =10 microM ATP induced Ca2+ oscillations that were accompanied by airway contraction. After approximately 1 min, the Ca2+ oscillations subsided and the airway relaxed. By contrast, > or =0.5 microM adenosine 5'-O-(3-thiotriphosphate) (nonhydrolyzable) induced Ca2+ oscillations in the SMCs and an associated airway contraction that...
متن کاملAcetylcholine-induced Calcium Signaling and Contraction of Airway Smooth Muscle Cells in Lung Slices
The Ca(2+) signaling and contractility of airway smooth muscle cells (SMCs) were investigated with confocal microscopy in murine lung slices (approximately 75-microm thick) that maintained the in situ organization of the airways and the contractility of the SMCs for at least 5 d. 10--500 nM acetylcholine (ACH) induced a contraction of the airway lumen and a transient increase in [Ca(2+)](i) in ...
متن کاملBiaxial distension of precision-cut lung slices.
The mechanical forces acting on lung parenchyma during (mechanical) ventilation and its (patho)physiological consequences are currently under intense scrutiny. Several in vivo and cell culture models have been developed to study the pulmonary responses to mechanical stretch. While providing extremely useful information, these models do also suffer from limitations in being either too complex fo...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-63225-y